The lyophilizer freeze-dried sample laboratory experiment steps, mainly divided into samples frozen, dry samples of two major steps!
Laboratory lyophilizer unit more than normal, gland, manifold, in situ optional, different devices, samples freeze-dried operation also have difference.
the following to ordinary lyophilizer, for example, said the lyophilizer sample experimental steps:
one, precool process operation
note: has a low temperature refrigerator users can save the step
(
1)
Open the refrigerator, to precooling device, when the temperature of the cold trap dropped to -
After below 40 ℃, can to precool material;
（
2)
The material in the material plate (
Liquid directly into the material plate, solid materials uniform or bottled on material tray)
；
（
3)
The material plate into the precool frame, probe into good contact with the material plate and material, in order to reduce the error of the sample temperature and real temperature;
（
4)
Put into cold trap the precool frame;
（
5)
Cover with insulation cover;
（
6)
Precool material parts to drop in temperature below the eutectic point of material, can maintain at about 1 small precool end, ready to enter drying stage.
2, drying process operation
(
1)
Remove precool frame from the cold trap;
（
2)
Remove the precool material in the drying rack;
（
3)
The drying rack into the cold trap, as shown;
（
4)
Check the sealing ring in good condition and without sundry under the premise of hood & other;
Organic glass tube & throughout;
；
（
5)
Tighten the water drain valve clockwise;
（
6)
Open the vacuum pump, open the vacuum gauge.
Vacuum degree in vacuum degree, the general drying process value & lt;
20 pa is normal.
（
7)
Dry after the first open & other;
Water (
Air intake)
Valve & throughout;
To shut down & other;
Vacuum pump & throughout;
。
Remove the organic glass tube, dry materials.
（
8)
Shut down & other;
Water (
Air intake)
Valve & throughout;
, devices for cream, cream after the open & other;
Water (
Air intake)
Valve & throughout;
Drainage, the device is wiped clean.
（
9)
Vacuum pump please cover when not working on the porosity, prevent dirt into.
note: a, in the whole process of drying machine shall not be shut;
b, because of differences in material properties, dry and precool time difference.
