Programmed temperature drop principle to
biological sample depends on the success of cryopreserved freezing rate, accurate temperature control and reasonable temperature gradient (
+ 40 ℃ to -
180℃)
。
When the temperature dropped to minus
At 180 ℃, the majority of biological samples can be transferred to the liquid nitrogen medium and long-term preservation.
in the process of cells cryopreserved, liquid water into ice, cell metabolism.
At the same time, water is lost, resulting in salt, the concentration of the metabolite changes in cells, causing the osmotic pressure imbalance, a direct impact on the recovery of cryopreserved cells.
At the same time, the freezing rate is too fast or too slow, easy to cause the cells to the loss of water or a large ice crystals, is not conducive to cell survival.
Through the use of programmable temperature drop, to choose the appropriate freeze-stored liquid, set the appropriate level of cooling rate, which can effectively overcome the above disadvantages.
The practice proved that (
After temperature compensation)
1 - per minute
3 ℃ freezing rate, it may improve the survival rate of the cryopreserved cells.
programmed temperature drop model parameters:
the main technical index being is
range of temperature control: 40 ℃ ~ -
180℃.
temperature accuracy: error & lt;
0.
5 ℃
the cooling speed: 0.
1 ℃ / min ~ 50 ℃ / points within the scope of the optional
heating speed: 0.
1 ℃ / min ~ 30 ℃ / min range of optional
the product type being fostered
type no.
technical reference number
TF -
PA-
I
range of temperature control: 40 ℃ ~ -
90℃;
Cooling speed: 0.
1 ℃ / min ~ 30 ℃ / min range of optional
TF -
PA
range of temperature control: 40 ℃ ~ -
110℃;
Cooling speed: 0.
1 ℃ / min ~ 40 ℃ / min range of optional
TF -
PA-
II
range of temperature control: 40 ℃ ~ -
150℃;
Cooling speed: 0.
1 ℃ / min ~ 40 ℃ / min range of optional
TF -
PA-
III
range of temperature control: 40 ℃ ~ -
180℃;
Cooling speed: 0.
1 ℃ / min ~ 50 ℃ / min range is optional