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Program temperature drop cryopreserved hematopoietic stem cells
by：CBFI
2020-10-01
The success of hematopoietic stem cell transplantation, it is important depends on hematopoietic stem cells cryopreserved and quick recovery and back to lose.
Program temperature drop of freeze-stored hematopoietic stem cell methods require the use of dimethyl sulfoxide (
DMSO)
10% hydroxyethyl starch (
他)
6% and human blood albumin (
HSA)
Ratio of 4% as a hematopoietic stem cell protective agent, according to certain proportion, temperature drop gradually cooling into the program, then transferred to -
80°
C deep cryogenic refrigerator, and finally in -
196°
C liquid nitrogen preservation, long time preservation of stem cells, under this condition can be achieved for decades, hematopoietic stem cells cryopreserved effect is good.
in recent years, research has confirmed that autologous hematopoietic stem cell transplantation to support high doses of chemotherapy, can improve the effect of sensitivity to chemotherapy of tumor; Allogeneic hematopoietic stem cell transplantation can effect a radical cure leukemia and malignant hematopoietic system disease. Hematopoietic stem cell transplantation as a safe and effective method, more and more accepted by the majority of medical workers and patients, the reasonable cryopreserved with hematopoietic stem cells in vitro and recovery are inseparable.
in recent years, research has confirmed that autologous hematopoietic stem cell transplantation to support high doses of chemotherapy, can improve the effect of sensitivity to chemotherapy of tumor; Allogeneic hematopoietic stem cell transplantation can effect a radical cure leukemia and malignant hematopoietic system disease. Hematopoietic stem cell transplantation as a safe and effective method, more and more accepted by the majority of medical workers and patients, the reasonable cryopreserved with hematopoietic stem cells in vitro and recovery are inseparable.
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